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Spectral flow cytometric tracking of mitochondria transfer in adipose tissue by age and diet

Unmixed fcs files for the stromal vascular fraction of epididymal (eWAT), inguinal (iWAT), and brown adipose tissue (BAT) in mtD2F/+AdipoqCre+/- and different ages or diets.  For a breakdown of samples and code, please see: <a href="https://github.com/ncborcherding/mtD2">https://github.com/ncborcherding/mtD2</a>. The full github repository at time of manuscript acceptance is also in the Zenodo repository under mtD2_GitHubRepo.zip. Antibody Panel:  <table align="center"> <tbody> <tr> <td> Laser </td> <td> Channel </td> <td> Antigen </td> <td> Fluorophore </td> <td> Final Dilution Factor </td> <td> Vendor </td> <td> Clone </td> <td> Catalog Number </td> </tr> <tr> <td> Violet </td> <td> V1 </td> <td> SiglecF </td> <td> BV 421 </td> <td> 1:400 </td> <td> BD Biosciences </td> <td> E50-2440 </td> <td> 562681 </td> </tr> <tr> <td> </td> <td> V2 </td> <td> CD44 </td> <td> Super Bright 436 </td> <td> 1:300 </td> <td> eBioscience </td> <td> IM7 </td> <td> 62-044-180 </td> </tr> <tr> <td> </td> <td> V3 </td> <td> CD11b </td> <td> Pacific Blue </td> <td> 1:300 </td> <td> BioLegend </td> <td> M1/70 </td> <td> 101224 </td> </tr> <tr> <td> </td> <td> V5 </td> <td> IL-33R⍺-biotin </td> <td> n/a </td> <td> 1:300 </td> <td> BioLegend </td> <td> DIH9 </td> <td> 145308 </td> </tr> <tr> <td> </td> <td> V5 </td> <td> Streptavidin (2°) </td> <td> BV 480 </td> <td> 1:300 </td> <td> BD Horizon </td> <td> n/a </td> <td> 564876 </td> </tr> <tr> <td> </td> <td> V7 </td> <td> MHC-II (I-A/I-E) </td> <td> BV 510 </td> <td> 1:300 </td> <td> BioLegend </td> <td> M5/114.15.2 </td> <td> 107636 </td> </tr> <tr> <td> </td> <td> V8 </td> <td> Ly6C </td> <td> BV 570 </td> <td> 1:400 </td> <td> BioLegend </td> <td> HK1.4 </td> <td> 128030 </td> </tr> <tr> <td> </td> <td> V10 </td> <td> KLRG1 </td> <td> BV 605 </td> <td> 1:300 </td> <td> BioLegend </td> <td> 2F1/KLRG1 </td> <td> 138419 </td> </tr> <tr> <td> </td> <td> V11 </td> <td> F4/80 </td> <td> BV 650 </td> <td> 1:300 </td> <td> BioLegend </td> <td> BM8 </td> <td> 123149 </td> </tr> <tr> <td> </td> <td> V13 </td> <td> Ly6G </td> <td> BV 711 </td> <td> 1:300 </td> <td> BioLegend </td> <td> 1A8 </td> <td> 127643 </td> </tr> <tr> <td> </td> <td> V14 </td> <td> CD3 </td> <td> BV 750 </td> <td> 1:300 </td> <td> BioLegend </td> <td> 17A2 </td> <td> 100249 </td> </tr> <tr> <td> </td> <td> V15 </td> <td> CD206 </td> <td> BV 785 </td> <td> 1:200 </td> <td> BioLegend </td> <td> C068C2 </td> <td> 141729 </td> </tr> <tr> <td> Blue </td> <td> B1 </td> <td> mtDendra2 </td> <td> n/a </td> <td> n/a </td> <td> n/a </td> <td> n/a </td> <td> n/a </td> </tr> <tr> <td> </td> <td> B2 </td> <td> TCRb </td> <td> AF 488 </td> <td> 1:300 </td> <td> BioLegend </td> <td> H57-597 </td> <td> 109215 </td> </tr> <tr> <td> </td> <td> B3 </td> <td> CD8 </td> <td> Spark Blue 550 </td> <td> 1:300 </td> <td> BioLegend </td> <td> 53-6.7 </td> <td> 100780 </td> </tr> <tr> <td> </td> <td> B8 </td> <td> CD45 </td> <td> PerCP </td> <td> 1:200 </td> <td> BioLegend </td> <td> 30-F11 </td> <td> 103130 </td> </tr> <tr> <td> </td> <td> B9 </td> <td> CD71 </td> <td> PerCP-Cy5.5 </td> <td> 1:200 </td> <td> BioLegend </td> <td> RI7217 </td> <td> 113816 </td> </tr> <tr> <td> </td> <td> B10 </td> <td> NKp46 </td> <td> PerCP-eFluor710 </td> <td> 1:200 </td> <td> eBioscience </td> <td> 29A1.4 </td> <td> 46-3351-82 </td> </tr> <tr> <td> Green </td> <td> YG1 </td> <td> TCRgd </td> <td> PE </td> <td> 1:300 </td> <td> BioLegend </td> <td> GL3 </td> <td> 118108 </td> </tr> <tr> <td> </td> <td> YG3 </td> <td> CD64 </td> <td> PE/Dazzle 594 </td> <td> 1:300 </td> <td> BioLegend </td> <td> X54-5/7.1 </td> <td> 139320 </td> </tr> <tr> <td> </td> <td> YG4 </td> <td> CD4 </td> <td> PE/Fire 640 </td> <td> 1:300 </td> <td> BioLegend </td> <td> GK1.5 </td> <td> 100482 </td> </tr> <tr> <td> </td> <td> YG5 </td> <td> CD62L </td> <td> PE-Cyanine5 </td> <td> 1:300 </td> <td> BioLegend </td> <td> MEL-14 </td> <td> 104410 </td> </tr> <tr> <td> </td> <td> YG7 </td> <td> CD11c </td> <td> PE-Cyanine5.5 </td> <td> 1:300 </td> <td> eBioscience </td> <td> N418 </td> <td> 35-0114-82 </td> </tr> <tr> <td> </td> <td> YG9 </td> <td> CD25 </td> <td> PE/Cyanine7 </td> <td> 1:200 </td> <td> BioLegend </td> <td> PC61 </td> <td> 102016 </td> </tr> <tr> <td> Red </td> <td> R1 </td> <td> CD9 </td> <td> APC </td> <td> 1:300 </td> <td> BioLegend </td> <td> MZ3 </td> <td> 124812 </td> </tr> <tr> <td> </td> <td> R2 </td> <td> CD127 </td> <td> AF 647 </td> <td> 1:200 </td> <td> BioLegend </td> <td> A7R34 </td> <td> 135020 </td> </tr> <tr> <td> </td> <td> R3 </td> <td> CD19 </td> <td> Spark NIR 550 </td> <td> 1:300 </td> <td> BioLegend </td> <td> 6D5 </td> <td> 115568 </td> </tr> <tr> <td> </td> <td> R4 </td> <td> CD90.2 </td> <td> AF 700 </td> <td> 1:300 </td> <td> BioLegend </td> <td> 30-H12 </td> <td> 105320 </td> </tr> <tr> <td> </td> <td> R5 </td> <td> CD5 </td> <td> BUV 737 </td> <td> 1:300 </td> <td> BD Horizon </td> <td> 53-7-3 </td> <td> 612809 </td> </tr> <tr> <td> </td> <td> R6 </td> <td> Viability dye </td> <td> Zombie NIR </td> <td> 1:1,000 </td> <td> BioLegend </td> <td> n/a </td> <td> 423106 </td> </tr> <tr> <td> </td> <td> R7 </td> <td> NK1.1 </td> <td> APC/Fire 750 </td> <td> 1:300 </td> <td> BioLegend </td> <td> PK136 </td> <td> 108752 </td> </tr> <tr> <td> </td> <td> R8 </td> <td> B220 </td> <td> APC/Fire 810 </td> <td> 1:300 </td> <td> BioLegend </td> <td> RA3-6B2 </td> <td> 103278 </td> </tr> </tbody> </table>

ShareScore

36/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship: 4
Harmonization: 4
Access: 16
Reuse readiness: 8
Engagement: 4