Multiplexed fluorescence imaging based on cycles, raw and processed data.

<p>This dataset was created from a larger acquisition in order to provide an example of reasonnable size, as a companion data set to the F1000Research paper preprint DOIXXX.</p> <ul> <li>The original raw data including metadata files are included in <strong>Microscope_Output.zip.</strong></li> <li><strong>Experiment.json</strong> and<strong> channelnames.txt </strong>are the ones generated by the acquisition software. They are the only files needed when starting from one of the processed data set below.</li> <li>The deconvolution obtained with the commercial software Microvolution is also provided in <strong>bu_deconvolution.zip.</strong> To start from Step 1(Extended Depth of Field) instead of Step 0 (deconvolution), unzip this file in your output directory and rename the folder bu_deconvolution to out.</li> <li>The extended field of view 2D images created from step 0 to step 2, provided for convenince in <strong>edfonly.zip</strong></li> <li>The final files generated by trhe Multiplex processor, including the segmentation mask , are provided in<strong> finaloutput.zip</strong>. These files can be used in a specific analysis software.</li> </ul> <p>&nbsp;</p>