FIGURE 3 in Genetic and morphological variation analyses of Glandirana rugosa with description of a new species (Anura, Ranidae)

FIGURE 3. Results of MIG-seq (Multiplexed ISSR genotyping by sequencing), showing unique position of East group. (A) A RAxML tree based on variable sites picked up from SNPs data. Numbers above branches represent bootstrap supports for ML inference. Number of each sample corresponds with those shown in the supplementary Table 1 deposited in Figshare (DOI: 10.6084/m9.figshare.20290599). Boxed numbers indicate the locality where we found both of West and se-Kyushu group of G. rugosa. (B, C) Plot of first against second (B) and third (C) principal scores of 397 alleles derived from SNPs data. Squares: East group of G. rugosa; Open diamonds: G. susurra; Closed diamonds: G. emeljanovi; Circles: Central, North, West, and seKyushu groups of G. rugosa. (D) Genetic structure in each individual of mitochondrial groups of Glandirana. Two primary genetic demes (K = 2) identified by the STRUCTURE analysis.