Transcriptional and metabolic changes in Candida albicans during yeast-to-hypha transition

<p>In this study hypha-associated transcriptional and metabolic changes were investigated in the opportunistich human pathogen <em>Candida albicans</em>. Three different strains were investigated the SC5314 wildtype and two filamentation-affected mutant strains <em>cph1</em>&Delta;<em>efg1</em>&Delta; and <em>hgc1</em>&Delta;. Three different hypha-inducing conditions were compared with two respective yeast conditions: SD minimal medium with 10% human serum or N-acetylglucosamin vs. SD minimal medium, and M199 pH7.4 vs. M199 pH4. Sampling time points were 0, 90 and 240 min. ON cultures were prepared in SD and re-inoculated in SD and grown to early log-phase prior to the experiment. Cells were collected, washed and used to inoculate the test medium at OD0.3. All cultures were generally maintained at 37&deg;C. Obtained samples were submitted for transcriptional profiling (RNA-Seq with 10 million reads per sample and 150 bp paired-ends) and metabolomics.</p> <p>Media list:</p> <p>SD: 2% glucose, 0.17% YNB without amino acids, 0.5% ammonium sulfate, pH 4.5</p> <p>HS - SD with 10 vol% human serum (Male, AB negative)</p> <p>GlcNAc - 2% <em>N</em>-acetylglucosamine, 0.17% YNB without amino acids, 0.5% ammonium sulfate, pH 4.5</p> <p>Medium 199 with Earle&#39;s salts and L-Glu&nbsp; buffered to either pH 4 or 7.4 by addition of 1/5 volume of 0.1&nbsp;M citric acid and 0.2&nbsp;M Na₂HPO₄ buffer</p>