Bacterial RNA Virus MS2 Exposure Increases the Expression of Cancer Progression genes in LNCaP Prostate Cancer Cell Line

<p><strong>Supplementary Figure 1. </strong>Network of protein-protein interactions in LNCaP cells according to their interaction with T4 and M13 phages (DNA phages). The network of protein-protein interactions in Homo Sapiens was constructed using the string protein-protein interaction network v 11.5 [35] with the overexpressed genes (highest confidence level setting) in LNCaP cells. Interactions of overexpressed protein genes were mapped with the highest confidence cut-off of (0.7-0.9). In the resulting protein association network, proteins are presented as nodes connected by lines with varying thicknesses representing the highest confidence level of (0.7-0.9). It is observed that there is strong experimentally determining evidence for the link between SRC and other overexpressed genes, including MAPKs and other integrins by adding more nodes until the highest confidence level of (0.7-0.9) among all overexpressed genes was obtained based on known interactions.</p> <p>&nbsp;</p> <p><strong>Supplementary Figure 2. </strong>Enrichr web server-based gene set enrichment analysis for overexpressed cancer progression genes in LNCaP cells treated with (A) MS2 and (B) T4 and M13 phages; which predicts caveolin-mediated endocytosis for MS2 bacterial virus (with a p-value of 0.000001399). Similarly, an integrin-mediated signaling pathway (with a p-value of 0.000007588) based on overexpression of most integrin genes and caveolin-mediated endocytosis (with a p-value of 0.000001799) were predicted for both T4 and M13 phages based on GO Biological process 2021.</p>