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Test data for the Large Genome Assembly tutorial

<p>A set of test data to use for the Galaxy Training Network tutorial, Large genome assembly.&nbsp;This data is publicly available in other sources, but has been combined here and subsampled for easier use in the tutorial.&nbsp;We do not claim ownership of this data - please see the full attribution to each of the data sources explained below.</p> <p><strong>Sequencing reads:</strong></p> <p>From the Snow gum: <em>Eucalyptus pauciflora</em>. From NCBI BioProject number: PRJNA450887; Paper: Wang W, Das A, Kainer D, Schalamun M, Morales-Suarez A, Schwessinger B, Lanfear R; 2020, doi: 10.1093/gigascience/giz160.</p> <p>From NCBI, three read files were imported into Galaxy for this tutorial: nanopore reads (SRR7153076), and paired Illumina reads (SRR7153045). For the test data set: these were randomly subsampled to 10% of the original file size, and reads mapping to related chloroplast gene sequences (rbcL sequence: accession KM360776.1; matK sequence: accession KT632904.1) were excluded.&nbsp;</p> <p>Files:&nbsp; Nanopore reads; Illumina reads, R1 and R2</p> <p><strong>Reference genome:&nbsp;</strong></p> <p><em>Arabidopsis thaliana.&nbsp;</em>Although this is not the same species as above, we can use it as an example for a comparison step in the tutorial.&nbsp;This has been downloaded from The Arabidopsis Information Resource at <a href="https://www.arabidopsis.org/index.jsp">https://www.arabidopsis.org/index.jsp</a>&nbsp;from Genes: Download: TAIR10 genome release: TAIR10 chromosome files: file TAIR10_chr_all.fas.gz. Then unzipped into a fasta file.&nbsp;</p>

ShareScore

36/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
4
Harmonization
4
Access
20
Reuse readiness
8
Engagement
0

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