Cross-species gRNA-design

<p>This dataset&nbsp;contains all scripts and data files required to run the&nbsp;<em><strong>Cross-species gRNA design </strong></em>shiny<em><strong>&nbsp;</strong></em>app&nbsp;created for the Perturb-seq project of the Hellmmann-Enard Lab. As part of this project, we plan to perturb a selection of transcription factors (TFs) using single-cell CRISPRi screens in primate iPS cells, infer gene regulatory networks (GRNs) based on the outcome of the perturbations, then quantitatively compare these GRNs aross species.&nbsp;</p> <p>During the experimental design, we selected TFs based on previous data that might be&nbsp;interesting to perturb&nbsp;and designed single-guide RNAs (gRNAs)&nbsp;to target them in the human and cynomolgus macaque genomes.&nbsp;The app aims to present all information about the TF selection and gRNA design in a structured and interactive manner.</p>