NGS in the clinical microbiology settings

<p>This dataset contains bam files from 16s rRNA targeted NGS sequencing from a variety of clinical specimens.&nbsp;The study included 55 specimens from 51 patients. We compared targeted NGS to Sanger sequencing in clinical samples submitted for Sanger sequencing.&nbsp;</p> <p>NGS:&nbsp;We sequenced the amplified V1-2 or V4 regions of 16s rRNA gene with Ion S5&trade; System (Thermo Fisher Scientific).&nbsp; Data were analyzed using the Ion Reporter bioinformatics Software pipeline (Thermo Fisher Scientific), using a threshold of 1000 mapped reads for designating significant pathogens. BAM files uploaded to the Ion Reporter were mapped to the Silva 138 SSU database.</p>