Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish
<p>To elucidate the mechanism underlying the aberrant monocyte/macrophage development of <em>zbtb14<sup>-/- </sup></em>mutant, GFP-positive cells were isolated from either wild-type <em>Tg(mpeg1.1:eGFP)</em> or <em>zbtb14 <sup>-/-</sup> //Tg(mpeg1.1:eGFP) </em>larvae at 2dpf by FACS. mRNA was extracted from sorted cells using RNeasy Micro (Qiagen, Manchester, UK) and mRNA libraries were constructed using NEBNext Ultra RNA Library Prep Kit for52 Illumina and sequenced under Illumina HiSeq X Ten with pair end 150bp (PE150). </p>
ShareScore
32/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 0
- Harmonization
- 12
- Access
- 12
- Reuse readiness
- 0
- Engagement
- 8
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