SNP amplicons results of 50 hybrid Chinook-Coho salmon

<p>These SNP panel results confirm the hybrid origin in 50 Chinook-Coho Salmon individuals. The SNP panel is composed of two amplicons and five diagnostic SNPs. DNA amplicons OkiOts_120255 and Oki_RAD41030 have SNP sites fixed for alternate base pairs in Chinook and Coho salmon (Beacham and Wallace 2019). The panel examined genotypes at one diagnostic position in OkiOts_120255 SNP: 113 (Reference=A, Variant=C) and four positions in Oki_RAD41030: 45 (TC), 51(CG), 195 (GA), and 198 (TG) called via Proton software Variant Caller®. The hybrid salmon were heterozygous for a Chinook and Coho haplotype at both SNP loci, confirming these as the parental species involved in the hybridization and consistent with all being F1 or higher order (F2 or back-cross) hybrid individuals. </p> <p>Beacham, T. D. &amp; Wallace, C. G. (2019). Salmon species identification via direct DNA sequencing of single amplicons. <i>Conservation Genetics Resources,</i> 1-7<i>. </i><a href="https://doi.org/10.1007/s12686-o19-01102-1">https://doi.org/10.1007/s12686-o19-01102-1</a>.</p>