Figure 1 from: Nilsson RH, Sánchez-García M, Ryberg M, Abarenkov K, Wurzbacher C, Kristiansson E (2017) Read quality-based trimming of the distal ends of public fungal DNA sequences is nowhere near satisfactory. MycoKeys 26: 13-24. https://doi.org/10.3897/mycokeys.26.14591

Figure 1 - Example of poorly trimmed sequences (sequence four and on) from the species Setosphaeria turcica. The 5' end of the alignment is shown, and the poorly trimmed sequences cover the last ~5 bases of SSU and the immediate start of ITS1. The topmost sequence is genome-derived, and sequences two and three are regular Sanger sequences retrieved from the INSDC from other studies than the one with the poorly trimmed sequences (sequences four and on). SeaView v. 4 (Gouy et al. 2010) was used to visualize the alignment.