Fig. 1 in Pre-treatment of canine plasma with heat, rather than acid, efficiently enhances Dirofilaria immitiS antigen detection

Fig. 1 Detection of Dirofilaria immitis antigen by ELISA for twofold-diluted plasma samples with and without treatment. A spectrophotometric OD reading at a wavelength of 415 nm was obtained for each sample using the microplate reader SpectraMax® i Series-Spectramax Id3. Filled black circle, untreated sera. Open blue circle, ICD-1 protocol. Filled blue circle, ICD-2 protocol. Open red circle, ICD-3 protocol. Filled red circle, ICD-4 protocol. The error was represented as mean ± 95% confidence interval