APPENDIX 3 in Fungal/bacterial syntrophy of glycerol utilization

APPENDIX 3. — Growth of Escherichia coli T.Escherich on M0 medium supplemented with glycerol. Escherichia coli DH5α was inoculated at position indicated by the blue dots (except at the one on the center where Podospora anserina (Rabenh.) Niessl would be inoculated when the plates contain the fungus, e.g. see Fig. 7) on the indicated media and the plates were incubated for 20 days at 27°C under constant illumination at which point photographs were taken. In these conditions, the growth of the E. coli colonies is readily visible after one week of incubation and starts to mask the blue dots, except on M0 that does not permit the growth of E. coli.