Healthy and cancer individuals from Long-read sequencing reveals aberrant fragmentation patterns and origins of circulating DNA in cancer

<p>Oxford Nanopore sequencing data from 61 samples described in manuscript "Long-read sequencing reveals aberrant fragmentation patterns and origins of circulating DNA in cancer"<br><br></p> <p>All data are aligned to UCSC analysisSet hg38 (https://hgdownload.soe.ucsc.edu/goldenPath/hg38/bigZips/analysisSet/), and use 0-based coordinates.</p> <p><strong>Level 2:</strong> In order to provide combined fragmentation and methylation information, Biscuit was used to create &ldquo;epiBED&rdquo; files (see Methods). epiBED files contain each read on a separate line, with each DNA methylation call on the read. epiBED files can be used for combined methylation/fragmentomic analysis, and are compatible with the CelFiE-ISH software that was used for cell of origin deconvolution.</p> <p>Creation of Biscuit EpiBED files:<br>Biscuit (https://huishenlab.github.io/biscuit/) v. 1.4.1-dev &nbsp;was used with the command &ldquo;biscuit epiread -M -b 0 -m 0 -a 0 -5 0 -3 0 -y 0.9 -L 1000000 hg38.analysisSet.fa&rdquo;, where the reference is the same UCSC reference genome used for alignment. Files are available in the Zenodo repository listed in Data Availability.</p> <p><strong>Level 3</strong>: DNA methylation BED files. BED files created by modkit (see Methods) provide one line for each CpG covered, and can be used for basic DNA methylation analysis.<br><br>Creation of modkit BED files:<br>BED files were created using modkit (https://github.com/nanoporetech/modkit) v. 0.1.5 with the command &ldquo;modkit pileup --cpg --combine-strands --ignore h --filter-threshold 0.9 --bedgraph".&nbsp;</p> <p>&nbsp;</p>