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Raw ONT pod5 data: Barcoded D2O Aminobacter P9b DNA

<p>Aminobacter P9b samples were incubated on a 100% D2O, 30% D2O and 0% D2O medium, respectively. Cells were harvested by centrifugation. Pellets were resuspended and DNA extracted. The DNA was barcoded using the ONTs native barcoding kit, prepared with the SQK-LSK109 protocol, and sequenced on a MinION with an r9.4.1 flowcell.</p> <p>For barcoding, we used the EXP-NBD114 Barcodes 14, 15, and 16:</p> <p>BC 14: 0% D2O<br>BC 15: 30% D2O<br>BC 16: 100% D2O</p>

ShareScore

32/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
4
Harmonization
8
Access
8
Reuse readiness
8
Engagement
4