Figure 6 in Shell formation in two species of bivalves: the role of mantle cells and haemocytes

Figure 6. Transverse section of outer margin of coat of Polititapes aureus, 20×, 40×; scale bars, 20μm, 50 μm. Epithelial cells immunoreactive for S100 (green) and 5-HT (red) are colocalized and evident (arrowheads). Haemocytes reactive for S100 and 5HT are notable and well colocalized in the epithelium and subepithelium (arrows). Epithelial cells immunopositive for iNOS and TLR2 are noticeable and well colocalized (arrowheads). Immunoreactive haemocytes that colocalize for iNOS and TLR2 are evident (arrows) in epithelium and subepithelium. In addition, some haemocytes are positive only for iNOS (*) others only for TLR2 (double arrows), suggesting a different hemocyte population with different function. The 'display profile' function of the confocal microscope confirms the colocalization.