Figure 4 in Taxonomic inflation and a reconsideration of speciation in the Andes: the case of the high-elevation tree frog Dendropsophus molitor (Anura: Hylidae)

Figure 4. MC1R coding region test for association with colour polymorphism. A, Haplotype network for MC1R including D. molitor (green, variegated, and brown) and O. histrionica (black and brown) individuals. Haplotypes of D. molitor did not clustered in association with colour pattern, whereas O. histrionica showed different haplotypes for brown and black dorsal background colour patterns. B, SNPs found in the 557 bp fragment of the coding region of MC1R amplified for D. molitor in comparison with O. histrionica. The alignment shows a 34 bp region (from 409 bp to 443 bp) of this gene. Each morphotype for D. molitor (green, variegated, and brown) and O. histrionica (black and brown) is represented by coloured vertical bars at the right of the alignment. Positions highlighted in a red box indicate Δ433 and C432A mutations responsible for differences in darker dorsal background colour patterns in O. histrionica (Posso-Terranova and Andrés 2017).