Fig 3 in Development of specific PCR assays for the detection of Cryptocaryon irritans

Fig 3 Evaluation of the sensitivity of the specific PCR assay for Cryptocaryon irritans (primer set P1/S15) by agarose gel electrophoresis. Lanes 1 and 13 represent a DNA size marker. Lane 2 represents DNA undiluted (450 ng/µl), and lanes 3 to 11 represent C. irritans DNA diluted for 1:50 (9 ng/µl), 1:100 (4.5 ng/µl), 1:200 (2.25 ng/µl), 1:400 (1.13 ng/µl), 1:800 (0.56 ng/µl), 1:103 (0.45 ng/µl), 1:104 (45 pg/µl), 1:105 (4.5 pg/µl), and 1:106 (0.45 pg/µl), respectively. Lane 12 represents no-DNA control