Figure 4 in Detection of the Trp-2027-Cys Mutation in Fluazifop-P-butyl-resistant Itchgrass (RottboelliO cochinchinensis) using High-Resolution Melting Analysis (HRMA)

Figure 4. High-resolution melting analysis (HRMA) for detection of mutation Trp-2027-Cys in Rottboellia cochinchinensis carboxyl-transferase domain of the acetyl-coenzyme A carboxylase gene conferring resistance to fluazifop-P-butyl. Three genotypes are included: wild type (homozygous TGG, susceptible), mutant homozygous (TGC, resistant), and artificial mutant heterozygous (TGG/TGC, possibly resistant). (A) Representative profiles of the melting curves (derivative melt curves), (B) normalized plot, and (C) difference plot using susceptible (wild type) as the reference genotype.