Comparative analyses of compatible and incompatible host-pathogen interactions provide insight into divergent host specialization of closely related pathogens

<p>The following animations accompany the manuscript "Comparative analyses of compatible and incompatible host-pathogen interactions provide insight into divergent host specialization of closely related pathogens". These animations are of confocal microscopy images of wheat leaves infected with isolates of&nbsp;<em>Zymoseptoria pseudodarliae&nbsp;</em>and&nbsp;<em>Z. ardabiliae,&nbsp;</em>at two infection stages (early and late), scrolling through different planes of focus into the wheat leaf. Two isolates of <em>Zymoseptoria pseudodarliae (</em>Zp13 and Zp72) and three isolates of&nbsp;<em>Zymoseptoria ardabiliae&nbsp;</em>(Za17, Za48, and Za94) on wheat, were screened in this study. Each isolate is expressing GFP in order to visualise hyphae.&nbsp;<em>Z. pseudodarliae&nbsp;</em>and&nbsp;<em>Z. ardabiliae&nbsp;</em>are avirulent on wheat, and infections do not pentrate beyond the stomata and into the wheat tissue. These differ from previously obtained images of&nbsp;<em>Z. tritici&nbsp;</em>(virulent on wheat), which penetrate into the wheat tissue (<a href="https://doi.org/10.1002/ece3.4724">https://doi.org/10.1002/ece3.4724</a>).</p>