Figures 1–9 in Oogenesis in Phragmatopoma (Polychaeta: Sabellariidae): Evidence for morphological distinction among geographically remote populations

Figures 1–9. Histology and Histochemistry in Phragmatopoma caudata of the SW Atlantic. Figures 1 and 2 Oogonia (Oo) and previtellogenic oocytes (Pv) of early vitellogenesis (Ev) associated with the intersegmental blood vessels (BV) for cytoplasmic prolongations (black star). Oocytes in advanced and late vitellogenesis are released into the coelomic cavity (white star). Oocytes in vitellogenesis have intensely acidophilic granules, and the ripe oocytes are less acidophilus (black arrows). Figure 2 depicts oocytes in late vitellogenesis occupying the entire coelom with a basophils vitelline envelope (white arrow). N = nucleus; H&E staining. Scales = 20 μm. Figures 3 and 4 Techniques used to visualize basic and total proteins, respectively. The previtellogenic and vitellogenesis (Ev) oocytes (Pv) have intense granules (large black arrow), and the ripe in late vitellogenesis (Lv) are less reactive (white arrow). The vitelline envelope is reactive to protein (small arrow). Scales = 20 μm. Figure 5 Technique for visualizing neutral polysaccharides with positive staining in both the previtellogenic oocyte cytoplasm and the vitellogenic oocyte granules. The reactivity disappears in the vitellogenic granules of ripe oocytes, but surrounding these is a noticeable positive staining (large black arrow). Star = oogonia with little reactivity to neutral polysaccharides. The egg envelope is reactive to PAS (small arrow). Scale = 20 μm. Figures 6 and 7 Absence of acid polysaccharides (pH 1.0 and 2.5), respectively, in oogonia and oocytes in P. caudata. Scales = 20 μm. Figures 8 and 9 Lipids stained by Sudan Black B. The oogonia are uniformly positive (small white arrow), while the oocytes during previtellogenesis and vitellogenesis have droplets in the cytoplasm (large black arrow). The yolk granules are reactive during vitellogenesis, while the staining is less intensive (large white arrows) in the mature oocytes. The vitelline envelopes have lipids (small black arrow). Scales = 20 μm.