Figure 3. Designing a in Omics in Weed Science: A Perspective from Genomics, Transcriptomics, and Metabolomics Approaches

Figure 3. Designing a metabolomics study. (A) The various approaches for performing a metabolomics experimental study. GC-MS, gas chromatography–mass spectrometry; HILIC-LC-MS/MS, hydrophilic interaction chromatography for liquid chromatography–tandem mass spectrometry; LC-MS/MS, liquid chromatography–tandem mass spectrometry. (B) The general metabolomics workflow. It involves formulating a biological question, setting up an experimental design to test the hypothesis, sample treatment and harvest, metabolite extraction, clean-up, chromatographic separation, identification, statistical validation, and functional interpretation.