Mutant Harmonia axyridis raw sequencing data of CRISPR/Cas9 induced mutations in the genes laccase2 and scarlet

<p><em>Harmonia axyridis</em> (Pallas), commonly known as the Asian lady beetle, is a native insect species of Asia that has been intentionally introduced to various regions for biocontrol purposes. However, its widespread presence beyond its original release sites suggests a high degree of invasiveness. In this study, we utilized the CRISPR-Cas9 approach to achieve precise genome editing in <em>H. axyridis</em>. Specifically, we targeted two genes in <em>H. axyridis</em>, <em>laccase2</em> and <em>scarlet</em>, knockdown of which orthologs in other insects showed visible phenotypic changes. The knockout <em>laccase2</em> resulted in an early-detectable phenotype but also in lethality. However, we successfully established a viable and genetically stable mutant colony by disrupting the <em>scarlet </em>gene, resulting in beetles with white eyes. Our findings contribute to the expanding knowledge of genetic manipulation in <em>H. axyridis</em> and provide insights into its potential for future research and practical applications for biocontrol and invasive species management.</p>