Extended rat miRNA repertoire

<p>Generally, <em>Rattus norvegicus'</em> miRNA repertoire falls short compared to the other rodent model organism,&nbsp;<em>Mus musculus.</em></p> <p>To extend the miRNA catalogue in <em>Rattus norvegicus,</em> we utilized Infernal v1.1 (<a href="https://doi.org/10.1093/bioinformatics/btt509" target="_blank" rel="noopener">Nawrocki and Eddy, 2013</a>) to derive potential rat miRNA candidates starting from all available mammalian miRNA families in <a href="https://www.mirbase.org/" target="_blank" rel="noopener">miRBase</a>. We utilized MIRfix (<a href="https://doi.org/10.1093/bioinformatics/btz271" target="_blank" rel="noopener">Yazbeck et al., 2019</a>) to curate the extended miRNA datasets automatically. Subsequent manual inspection and curation of miRNA alignments resulted in a reliable and comprehensive update to the rat miRNA annotation.</p> <p>Key facts of the extended miRNA repertoire</p> <ul> <li>342 miRNA families (40 novel families)</li> <li>549 miRNA sequences (56 novel miRNAs)</li> <li>&nbsp;11 corrected annotated miRNAs</li> </ul> <h3>European Nucleotide Archive</h3> <p>The 56 novel sequences not listed in miRBase before have been submitted to the European Nucleotide Archive at EMBL-EBI.<br>They are accessible with the accession numbers OZ078105 - OZ078160.<br>The sequences will be permanently available from the ENA browser at http://www.ebi.ac.uk/ena/data/view/&lt;ACCESSION NUMBERS&gt;.</p> <p>An overview of all sequences is given here: <a href="http://www.ebi.ac.uk/ena/data/view/OZ078105-OZ078160" target="_blank" rel="noopener">http://www.ebi.ac.uk/ena/data/view/OZ078105-OZ078160</a>.</p>