Fig. 2. After 73 in Osmotic induction marking with Alizarin Red S on juveniles of pejerrey, Odontesthes bonariensis (Atherinopsidae)

Fig. 2. After 73 days post-treatment, clear marks were observed for both marking procedures, in otoliths (Figs. 2a, b), scales (Figs. 2c, d) and caudal fin rays (Figs. 2e, f). The intensity of the signal in scales was higher in fish marked with OI+ARS (Fig. 2d). After 385 days, only marks in caudal fin rays were observed in fish treated with OI+ARS (Fig 2g). All control fish structures and, otoliths and scales for 385 days post- immersion showed no marks under fluorescence microscope (Fig. 2h). a, c, e: ARS treatment; b, d, f, g: OI+ARS treatment. Scale bar = 200 µm.