Fig. 1 in 17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxyprogesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae)

Fig. 1. Radiochromatogram after TLC analysis of spermiating testes of Micropogonias furnieri males 1, 2 and 3 after toluene/cyclohexane 50/50 v/v (system I) and benzene/acetone 80/20 v/v (system II). The TLC zones further analyzed by HPLC are shown as A, B and C. The peak of precursor is shown as D. The authentic standards used were: 1 = 20-dihydrocortisone; 2 = 17,20,21-P; 3 = 17,20α-P; 4 = 11KT; 5 = 17,20ss-P; 6 = 11ssOH-A; 7 = Ad; 8 = testosterone; 9 = 17-P; 10 = A.