Figure 2 in Saccharomyces cerevisiae OS303 expression of an alkaline protease from a newly isolated Bacillus subtilis D9

Figure 2. Analysis cloning vector construction containing alkaline protease gene with restriction enzymes to confirm the integrity of the PCR product of alkaline protease gene and to design the appropriate primers to construct the expression vector. By the enzyme combination of NheI- NdeI- BamHI restriction enzymes showed two bands of approximately 3000bp and ~1300bp must be shown as alkaline protease gene. Lane 1: DNA size marker hyperladder I; Lane 2, 3, 4, and 5: Digested sample of TA Cloning vector construction containing alkaline protease with enzyme SpeI, EcoRI, XbaI, and NheI- NdeI- BamHI respectively.