Fig. 4 in Successful transcription but not translation or assembly of Solenopsis invicta virus 3 in a baculovirus-driven expression system

Fig. 4. Quantitative PCR (absolute) results evaluating transcript production of SINV-3 by AcSINV-3-infected Sf21 cells. RNA preparations from AcSINV-3 (2 rep- licates, Ci and Di) were treated with DNase I, reverse transcribed, and amplified by qPCR. Results were compared with a series of plasmid constructs containing a portion of the SINV-3 genome (102–109 genome equivalents). The region amplified was at the 3'-most end of ORF2 (see Fig. 2). No amplification was detected in polyhedrin-negative AcRP23.lacZ preparations.