Fig. 4 in A retrospective study of Babesia macropus associated with morbidity and mortality in eastern grey kangaroos (Macropus giganteus) and agile wallabies (Macropus agilis)

Fig. 4. Phylogenetic tree of 18S ribosomal RNA (18S rRNA) gene sequences of eastern grey kangaroo and agile wallaby Babesia and other piroplasms that are in the GenBank nucleotide database. For each sequence, the GenBank GI number is followed by the species name. The representative Babesia isolates from eastern grey kangaroos and an agile wallaby in this study are shown with a - and a ♦ respectively. Evolutionary history was inferred using the Maximum Likelihood method based on the Tamura 3-parameter model. The tree with the highest log likelihood (−1820.5242) is shown. Initial tree for the heuristic search was obtained automatically as follows. When the number of common sites was <100 or less than one fourth of the total number of sites, the maximum parsimony method was used; otherwise, BIONJ method with MCL distance matrix was used. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The scale bar represents the number of substitutions per nucleotide. All positions containing gaps and missing data were eliminated. Evolutionary analyses were conducted in MEGA6 (Tamura et al., 2011).