Figure 1 in Severe glomerular disease in juvenile grey snapper Lutjanus griseus L. in the Gulf of Mexico caused by the myxozoan Sphaerospora motemarini n. sp.

Figure 1. Morphology and histopathology of Sphaerospora motemarini n. sp. in grey snapper, Lutjanus griseus. (A) Mature spore in fresh kidney smear showing thickened suture at apical part (transparent arrowhead), two prominent bulges at posterior part (black arrowheads) and valve surface ornamentation (detailed in D) at the posteriolateral part of the spore; bar = 10 µm. (B) DAPI nuclear staining showing polysporoplasmic nature of spore (4 out of 6 sporoplasms visible in plane of image); bar = 10 µm. (C) Pseudoplasmodium containing two croissant-shaped sporoplasms (only visible around left spore) and two spores; bar = 10 µm. (D) SEM showing spore surface ornamentation in the shape of pits providing an opening to a multilayered canal system; bar = 5 µm. (E) Fresh smear of kidney showing early plasmodial stages (arrowheads) in a glomerulus; bar = 20 µm. (F–H) Histological sections stained with H&E; bar = 50 µm (F). Histopathological changes showing massive enlargement of renal corpuscles, with arrows in small insert (bottom left) indicating uninfected corpuscles of normal size in an uninfected kidney; bar = 50 µm. (G) Thickening of Bowman's capsule (arrows) and proliferation of mesangial cells; bar = 50 µm. (H) Engulfment of a spore by a melanin-rich macrophage (brown color); bar = 10 µm.