Fig. 1 in Fig. 2 in Garypus sanasai Lin, Huang & Chang 2022, sp. nov.

Fig. 1. Western blot analysis of PP1-C, PNUTS, and PP4-C in silkworm eggs. Following SDS-PAGE and immunoblotting, silkworm egg extracts (a quarter of one egg for each lane) were probed with anti-PP1α (PP1), anti-PNUTS (PNUTS), anti-PP4-C (PP4), and anti-HSP 90 (HSP) antibodies. (A) Results from day 4. D4, extracts from day 4 diapause eggs; H4, extracts from eggs whose diapause was prevented by HCl (4 days after HCl treatment). (B) Results from day 7. D7, extracts from day 7 diapause eggs; H7, extracts from eggs whose diapause was prevented by HCl (7 days after HCl treatment). Results shown in the left panel are representative of four independent experiments. Each protein band was quantified and normalized to the level of HSP in the right panel. Asterisks indicate significant differences compared to respective diapause eggs (by Student's t-test, ** p <0.01).