Fig. 5 in Fig. 2 in Garypus sanasai Lin, Huang & Chang 2022, sp. nov.

Fig. 5. Western blot analysis of protein levels of PP1-C, PNUTS, and PP4-C in eggs in which diapause had been terminated by chilling of diapausing eggs at 5°C for 70 days and then transferred to 25°C. Egg lysates from each stage after being transferred to 25°C were prepared and subjected to an immunoblot analysis with anti-PP1α (PP1), anti-PNUTS (PNUTS), anti-PP4-C (PP4), and anti-HSP 90 (HSP) antibodies. Lysates from a quarter of one egg were used for each lane. Molecular weight markers are shown on the right side of the gel (A). Results shown in the upper panel are representative of four independent experiments. Quantified protein levels relative to HSP were standardized to levels from the first day after transfer to 25°C. Different letters above the bars indicate significant differences (ANOVA followed by Tukey's test).