Fig. 2 in Fig. 2 in Garypus sanasai Lin, Huang & Chang 2022, sp. nov.

Fig. 2. Effect of HCl treatment on protein levels of PP1-C, PNUTS, and PP4-C during the first 8 days after treatment. Diapause-destined eggs that had been incubated at 25°C for 20 h after oviposition were treated with HCl and then incubated at 25°C. Egg lysates from each stage were prepared and subjected to an immunoblot analysis with anti-PP1α (PP1), anti-PNUTS (PNUTS), anti-PP4-C (PP4), and HSP 90 (HSP) antibodies. Lysates from a quarter of one egg were used for each lane. Molecular weight markers are shown on the right side of the gel (A). Results shown in the upper panel are representative of four independent experiments. Quantified protein levels relative to HSP were standardized to levels from the first day after HCl treatment. Different letters above the bars indicate significant differences (ANOVA followed by Tukey's test).