Fig. 5 in Branchinecta Muller 1788

Fig. 5. Light microscope observation of leukocytes from sea turtles studied in Taiwan. Examples of staining properties unsuitable for heterophil toxic change diagnosis. A–C: Examples of failure to demonstrate appropriate staining properties for reliable interpretation of cellular changes. During the 100-heterophil count, the higher the percentage of these kinds of cell seen on a stained slide, the less diagnostic the slide; A: granule coalescence caused the loss of cytoplasmic detail, therefore changes in the granule number/staining properties/size and shape could not be reliably assessed, DiffQuik stain; B: overstaining caused abnormal coloration of the cell, therefore cytoplasmic basophilia and changes of the granule staining properties could not be reliably assessed, Diff-Quik stain; C: overstaining caused abnormal coloration of the granules, therefore changes in the granule staining properties could not be reliably assessed, Liu's stain.