Figure 1 Symptoms seen on peanut leaves inoculated with P. arachidicola conidia suspensions.

<p><strong><span>Figure 1</span></strong><span> Symptoms seen on peanut leaves inoculated with <em>P. arachidicola</em> conidia suspensions. The selected peanut leaves were spray inoculated with <em>P. arachidicola</em> conidia suspensions and their phenotypes were observed at 0, 7, 14, and 21dpi.</span></p> <p><strong><span>Figure 2 </span></strong><span>Gene characterization and analysis for differential expressed genes. (A). Venn dialog of expressed genes from genome analysis. Venn diagrams depict the numbers of expressed genes in peanut leaves that were infected by <em>P. arachidicola </em>at 7 (JZ7), 14 (JZ14), 21(JZ21) dpi. The numbers indicate the counts of common or unique expressed genes. (B). Heatmap of DEGs (differentially expressed genes). Red spots mean up-regulated genes; blue indicates down-regulated genes. Blue spots indicate down-DEGs, Red spots indicate up-DEGs. In the red-blue scale, darker red colors indicate the most upregulated and the darker blue the most downregulated genes. (C). Go analysis for gene functional classifcation shows the number of genes involved in the biological process, cellular component and molecular function, respectively. (D). KEGG pathway enrichments of DEGs. The panel shows 20 enriched KEGG terms for DEGs responding to <em>P. arachidicola </em>infection. The legend is shown on the picture.</span></p> <p><strong><span>Figure 3</span></strong><span> Analysis of IFS protein function (A). Functional domains, phylogenetic analysis, and multiple sequence alignment of the two AhIFS proteins. (B). Functional domain schematic for the AhIFS proteins. The predicted functional domains (http://smart.embl-heidelberg.de/) indicated that both proteins contained P450 domains. (C).Phylogenetic tree analysis of the AhIFS orthologs. Close orthologs from <em>Arachis hypogaea</em> and other plants, including MpIFS1 (<em>Mucuna pruriens</em>, RDX63227.1), PsIFS1 (<em>Pisum sativum</em>, AAQ10282.2), GmIFS1 (<em>Glycine max</em>, NP_001236022.1), and GsIFS1 (<em>Glycine soja</em>, ACA81484.1), were used for IFS protein sequence alignment. An amino acid identity of 100% is indicated by a black background; amino acids with conservation exceeding 50% are in black on a gray background; and similar amino acids are in white on a gray background.</span></p> <p><strong><span>Figure 4 </span></strong><span>(A).The flavonoids and isoflavones biosynthesis pathways. Enzymes are indicated in uppercase letters. PAL, phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate: CoA ligase; CHS, chalcone synthase; CHI, chalcone isomerase; IFS, isoflavone synthase; The expression of IFS genes refers to the expression of genes at three infection time points(7,14,21day) in the transcriptome. (B, C).Transcriptional expression of AhIFS genes at the infection stage. The y- and x-axes represent the relative expression levels and time course for <em>P. arachidicola</em> infection, respectively. Mean values and standard deviations (SDs) were obtained from three biological and three technical replicates. Error bars are SDs; time points are 0, 7, 14, and 21hpi; and asterisks indicate that the expression level was significantly different from the control (*P&lt; 0.05, **P&lt; 0.01).</span></p> <p><strong><span>Figure 5</span></strong><span> Overexpression of AhIFSs boosts resistance of <em>N. benthamiana </em>to <em>P. parasitica</em>. (A). Representative photographs of <em>N. benthamiana </em>leaves under infection. The constructs, pBINPLUS:AhIFSs and pBINPLUS:GFP, were transiently expressed in <em>N. benthamiana </em>leaves. (A). tumefaciens-mediated transient expression of CK (right) and AhIFSs (left) fusion proteins in <em>N. benthamiana </em>leaves. The infiltrated leaves (after 48 h) were inoculated with <em>P. parasitica </em>zoospores, and photographed at 36 hpi and48 hpi, respectively. (B). Lesion diameters (mm) of <em>N. benthamiana </em>leaves. The data was calculated from three independent biological replicates using at least eight leaves each (** P &lt; 0.01 compared with GFP; Dunnett&rsquo;s test).</span></p> <p><strong><span>Figure 6</span></strong><span> Position of target sites on the <em>AhIFS1</em> and <em>AhIFS2</em> genes, and identifcation of the mutant lines produced using the CRISPR/Cas9 system. (A). CRISPR/Cas9 sgRNA-1 and sgRNA-2 target the first exon of <em>AhIFS1</em> and <em>AhIFS2</em> genes.<span>&nbsp; </span>The ATTG is the initial recognition motif</span><span>,</span><span>and the protospacer adjacent motif (PAM) is underlined in red. Gray boxes, blue boxes and black lines indicate UTRs, exons and introns, respectively. (B). The edited sequences at the target site are shown in HY23 and mutation lines. The blue nucleotides indicate SNP in the mutant <em>ahifs1-5/16</em>, <em>ahifs2-5/11</em></span><span>,</span><em><span>ahifs2-26</span></em><span> and the blue &lsquo;-&rsquo; indicates the deletions between HY23 and mutant ahifs1-21. The target sequence is shown by the red underline, and nucleotides marked in red represent PAM. (C). Relative expression of <em>AHIFS</em> genes in the mutant. (D). Knockout of AhIFSs genes reduce its disease resistance. The symptoms of peanut mutant leaves inoculated with <em>P. arachidicola </em>conidia suspensions. The detached leaves of peanut were sprayed inoculation with <em>P. arachidicola </em>conidia suspensions and phenotypes were observed at 14 dpi.</span></p>