Fig. 1. A in A novel quantitative real-time PCR diagnostic assay for seal heartworm (Acanthocheilonema spirocauda) provides evidence for possible infection in the grey seal (Halichoerus grypus)

Fig. 1. A: graphical representation of cluster 20 (C20). B: representation of the selected contig and the sequence used to design the C20 quantitative real-time PCR assay. The forward primer is in bold, the reverse primer is indicated by a dotted underline, and the double-quenched probe is underlined.