Fig. 2 in Close relationship of Plasmodium sequences detected from South American pampas deer (Ozotoceros bezoarticus) to Plasmodium spp. in North American white-tailed deer

Fig. 2. Phylogenetic relationships of Plasmodium sequences from Brazilian pampas deer within Haemosporidia. The tree was constructed using ∼3.4 kb of partial mitochondrial nucleotide sequences by the maximum likelihood (ML) method based on the GTR + I + G model. Bootstrap values (BV) for ML with 1000 replicates of ultrafast bootstrap analysis and Bayesian posterior probability (BPP) are indicated for each internal branch. The compositions of collapsed clades are Leucocytozoon (L. fringillinarium, L. majoris, and L. sabrasezi); Haemoproteus and Parahaemoproteus (Haemoproteus sp. jb1.JA27, Haemoproteus sp. jb2.SEW5141, and Parahaemoproteus vireonis); and bird, lizard and non-ungulate mammalian Plasmodium (P. gallinaceum, P. relictum, P. juxtinucleare, P. lutzi, P. floridense, P. mexicanum, P. falciparum, P. vivax, P. malariae, P. ovale, P. coatneyi, P. cynomolgi, P. fieldi, P. gonderi, P. inui, P. knowlesi, P. fragile, P. simiovale, P. simium, P. hylobati, P. reichenowi, P. billicollinsi, P. billbrayi, P. berghei, P. chabaudi, P. vinckei, and P. yoelii). Mitochondrial DNA sequences (including cytb and cox1) used in this study were listed in the Supplementary Table S1 of Templeton et al. (2016a). Nucleotide sequences of Plasmodium sp. in the North American white-tailed deer was based on Table S4 of Martinsen et al. (2016). Nucleotide positions containing indels or undetermined nucleotides, or those where the alignment was not clearly made were excluded. Nucleotide positions corresponding to the P. falciparum mtDNA (NC_002375.1) 974–1502, 1509–1576, 1578–1628, 1637–1678, 1698–1760, 1762–1769, 1774–1800, 1806–1831, 1834–1867, 1870–1909, 1914–2031, 2050–3474, and 3486–4444 were used. The length for the substitutions/site (0.04) is indicated.