Hypoosmotic solutions affecting a monolayer of neonatal rat ventricular cardiomyocytes

<p><span>Live cell imaging was conducted with </span><span>Image ExFluorer (Live Cell Instrument, South Korea) </span><span>with an integrated environmental control system including CO</span><span><span>2</span></span><span>, O</span><span><span>2</span></span><span>, humidity control and temperature. The prepared samples were placed in 1T solution in the incubator system at 37&deg;C and 5% CO</span><span><span>2</span></span><span>. Images were taken every 1 minute for 90 minutes with a phase contrast plan semi-apochromatic correction 10x objective (Nikon Instruments, Japan) and 5.5MP sCMOS camera (Nikon Instruments, Japan). After 5 minutes of incubation in 1T solution, the solution was changed to 0.75T or 0.5T. No external stimulation was used in order to stop the spontaneous contraction and register only changes in cell volume. </span></p>