Fig. 1 in Asymbiotic seed germination and in vitro propagation condition in Calanthe discolor Lindl.

Fig. 1. Developmental stages of asymbiotically cultured Calanthe discolor seeds. (A) surface of seed coat without NaOCl treatment; (B) surface of seed coats after 1% NaOCl treatment for 30 min by SEM (arrows: perforation); (C) hyaline embryo, seed coatintact; (D) embryos swollen after 2 weeks of culture; (E) swellled embryos present rhizoids after 6 weeks of culture; (F) appearance of protomeristem and rhiz­ oid elongation after 7 weeks culture; (G) appearance of chlorophyllous protomeristem after 10 weeks of culture; (H) shoot formation via the shoot axis; (I) The well­developed plantlets were removed from POM medium; Scale bars: A, B = 100 μm, C­G = 1.0 mm, H, I = 25.0 mm.