Dataset of Axon Segmentation and Centerlines, Acquired using a Two-photon Microscope in the Live Mouse Cortex

<p>This dataset contains 20 images of real axons that were published previously in Bass et al (2017). This subset dataset has been added labels&nbsp;of binary images for segmentation of the axons, and of centerline for tracing of the axons.</p> <p>We provide the following:</p> <ul> <li>Images-MAX: 2D images of axons (.png)</li> <li>Images-TIFF: 3D images of axons (.tiff)</li> <li>Labels-binary: Manual&nbsp;segmentation of the axons as binary images (.png)</li> <li>Labels-tracing: Manual tracing of the axons (.swc)</li> </ul> <p>This data was collected in the live mouse cortex, using a two-photon microscope,&nbsp;with a 40x&nbsp;objective, at zoom 4, and with a resolution of 512 &times; 512 pixels, 0.147&nbsp;<em>&mu;</em>m per pixel for the&nbsp;<em>x</em>,&nbsp;<em>y</em>&nbsp;planes, and 1&nbsp;<em>&mu;</em>m for the&nbsp;<em>z</em>&nbsp;plane.&nbsp;.&nbsp;</p> <p><strong>Please cite the following papers when using this dataset:</strong></p> <p>T. Dai, M. Dubois, K. Arulkumaran, J. Campbell, B. Billot, C. Bass, Z. Uslu, V. De Paola, C. Clopath, and A. A. Bharath. Deep reinforcement learning for subpixel neural tracking.&nbsp;<em>Medical Imaging with Deep Learning</em>.&nbsp;2019.</p> <p>Bass C, Helkkula P, De Paola V, Clopath C, Bharath AA. Detection of axonal synapses in 3D two-photon images. Giniger E, ed. <em>PLoS ONE</em>. 2017;12(9):e0183309. doi:10.1371/journal.pone.0183309.</p>