Expression of NSD3short-3xFLAG in Multiple Cell Lines

<p><strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong></p> <p><strong>Exp018&nbsp;Objective:</strong>To generate cell lines stably expressing NSD3-Short-3xFLAG in relevant cell backgrounds. These cell lines can be used to assay phenotypes associated with over-expression and to study NSD3-short protein-protein&nbsp;/ protein-chromatin interactions. The lentiviral vectors used here have been modified by replacing an IRES bicistronic element with a T2A self-cleaving peptide sequence (cloning described in exp-016) for expression of NSD3short-3xFLAG and a puromycin selection marker.</p>