Transfection of NSD3-tareting siRNA in H1299 Cells
<p><strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong></p> <p><strong>Exp019 Objective: </strong> I have identified a putative phenotype in H1299 cells upon overexpression of the NSD3 short<br> isoform. I am also interested in any phenotype resulting from decreased amounts of NSD3 in cells. To<br> do so, I am first testing conditions for RNAi-mediated knockdown of NSD3 in H1299 cells by treating cells<br> with several concentrations of siRNA and evaluating knockdown by western blotting</p>
ShareScore
32/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 4
- Harmonization
- 4
- Access
- 16
- Reuse readiness
- 8
- Engagement
- 0
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