Quantification of E-cadherin Expression in H1299 NSD3 Knockdown Cells

<p><strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong></p> <p><strong>Exp023 Objective:&nbsp;</strong>Having observed downregulation of E-cadherin protein levels in response to NSD3 overexpression in H1299 cells (exp021), I was next interested in testing the consequences of NSD3 knockdown. To do so, I transfected H1299 cells with siRNA targeting NSD3, both short and long isoforms, and assayed E-cadherin expression by western blotting. &nbsp;</p>