The process of HDAC11 Assay Development: Buffer optimization again

<p>The pH range used earlier was expanded for further optimization to check if the activity could improve.</p> <p>&nbsp;</p> <p><strong>Note: </strong>1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%).</p> <p>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.</p>