AFLP dataset and sequence alignments from a study of Rhododendron ferrugineum across the whole species range.

<p>STRUCTURE DATASET</p> <p>The raw data are in the files:</p> <ol> <li>Rfe_KAR_AAT-CAC2_2017-10-18-16-55-16.zip</li> <li>Rfe_KAR_ATC-CAC_2017-10-25-12-39-28.zip</li> <li>Rfe_KAR_ATG-CTG2_2017-10-24-09-40-25.zip</li> </ol> <p>Population data: populations.txt</p> <p>The final structure file: Rfe.str</p> <p>We analysed the obtained results in GeneMapper (Applied Biosystems) with default settings for AFLP analysis.&nbsp;The combinations of selective starters amplified the following numbers of polymorphic markers: AAT-CAC=127 (mean=40,4; SD=8,1), ATC-CAC=119 (mean=35,3; SD=5,4), ATG-CTG=144 (mean=44,4; SD=5,0). The blank samples yielded 0, 6 and 5 markers, respectively, which were removed from the final data matrix. We also removed the markers present in only one individual. Three samples (one from each of the populations L22, K27 and S05)&nbsp;failed to amplify in one of the selective primer reactions and were thus removed from the final matrix. The estimated error rate was 2.87% and the final matrix comprises of 87 individuals and 254 polymorphic loci.</p> <p>SEQUENCE ALIGNMENTS</p> <ol> <li>Rfe_ITS_align.fas</li> <li>Rfe_rpl32-trnL_align.fas&nbsp;</li> <li>Rfe_RPS12-RPL20_align.fas&nbsp;</li> <li>Rfe_trnfM-trnS_align.fas&nbsp;&nbsp; &nbsp; &nbsp;</li> <li>Rfe_trnLF_align.fas&nbsp;</li> </ol>