Plant scoring trial: U.Nottm_2018_RIPR_leaf_nitrate_concentration

This study makes use of the Renewable Industrial Products from Rapeseed (RIPR) diversity population of inbred lines of Brassica napus genotypes Thomas et al., 2016. BMC Plant Biology 16, 214). A subset of 383 genotypes were selected, comprising 169 winter-, 123 spring-, and 11 semiwinter-oilseed rape (OSR), 27 swede, six fodder, three kale and 44 of unspecified growth types. All plant growth took place at the Sutton Bonington Campus of the University of Nottingham (52°49'58.9"N, 1°14'59.2"W) in compost in a designed experiment in a polytunnel Leaves were sampled from all plants at the rosette stage (typically 6-8 true leaves showing) approximately four months after sowing. Typically three leaves were taken from each plant and freeze dried for 48-60 hours. Leaves were then homogenised in liquid N2 using a pestle and mortar and stored at -80°C prior to analyses. Leaf NO3- concentration was measured by ion chromatography (IC). This work was supported by the Biotechnology and Biological Sciences Research Council [grant number BB/L002124/1], (BBSRC, United Kingdom), Renewable Industrial Products from Rapeseed (RIPR) Programme to Ian Bancroft.