Br soaked lysozyme datasets collected with the mixed scheme implemented in HITO in the ZOO system

<p>Bromide soaked lysozyme crystals were used as standard crystals for evaluation of the automated data collection, ZOO, developed at SPring-8 BL32XU.</p> <p>Crystals those sizes ranged 10-100 &micro;m were cryocooled together in the same cryoloop and processed &#39;mixed scheme&#39; implemented in the ZOO system.</p> <p>Beam size : 10(H) x 15(V) &micro;m²</p> <p>After 2D raster scanning the loop, HITO detects 1&nbsp;crystals for full rotation helical, 7 crystals for partial helical, and 62 crystals for multiple small wedge schemes, respectively. A full rotation helical, partial helical and small wedge datasets contain total oscillation of 60 deg, 40 deg and 5 deg. respectively.&nbsp;</p> <p>The crystals belong&nbsp;to space group P4₃2₁2 with mean unit cell parameters a=b=79.1, c=37.5.</p> <p>Data was processed up to&nbsp;1.50 &Aring; resolution in the published result (Hirata et al. in preparation) using KAMO.</p> <p><em><strong>&lt;Available data&gt;</strong></em></p> <ul> <li># Beamline: &nbsp; &nbsp; SPring-8 BL32XU</li> <li># wavelength&nbsp; &nbsp; 0.9000 A</li> <li># Detector :&nbsp; &nbsp; EIGER X 9M</li> <li># ZOO mode: mixed scheme (HITO)</li> <li>#####################################################</li> <li># Multiple small wedge datasets</li> <li># &#39;CPS1991-05-multi_master.h5&#39; manages 62 datasets</li> <li>#####################################################</li> <li># 5.0 deg. / datasets</li> <li># 0.1 deg. / frame</li> <li>#&nbsp; 50 frames / datasets</li> <li># 100 frames in each data file</li> <li>#####################################################</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000001.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000002.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000003.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000004.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000005.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000006.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000007.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000008.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000009.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000010.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000011.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000012.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000013.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000014.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000015.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000016.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000017.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000018.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000019.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000020.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000021.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000022.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000023.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000024.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000025.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000026.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000027.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000028.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000029.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000030.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_data_000031.h5</li> <li>hito-CPS1991-05//data/CPS1991-05-multi_master.h5</li> </ul> <p>&nbsp;</p> <ul> <li>#####################################################</li> <li># &#39;cry-00-??_master.h5&#39; are for &#39;partial helical&#39; datasets</li> <li>#####################################################</li> <li># 40.0 deg. / datasets</li> <li>#&nbsp; 0.1 deg. / frame</li> <li>#&nbsp; 400 frames / datasets</li> <li>#&nbsp; 100 frames in each data file</li> <li>#####################################################</li> <li>hito-CPS1991-05//data/cry-00-00_data_000001.h5</li> <li>hito-CPS1991-05//data/cry-00-00_data_000002.h5</li> <li>hito-CPS1991-05//data/cry-00-00_data_000003.h5</li> <li>hito-CPS1991-05//data/cry-00-00_data_000004.h5</li> <li>hito-CPS1991-05//data/cry-00-00_master.h5</li> <li>hito-CPS1991-05//data/cry-00-01_data_000001.h5</li> <li>hito-CPS1991-05//data/cry-00-01_data_000002.h5</li> <li>hito-CPS1991-05//data/cry-00-01_data_000003.h5</li> <li>hito-CPS1991-05//data/cry-00-01_data_000004.h5</li> <li>hito-CPS1991-05//data/cry-00-01_master.h5</li> <li>hito-CPS1991-05//data/cry-00-02_data_000001.h5</li> <li>hito-CPS1991-05//data/cry-00-02_data_000002.h5</li> <li>hito-CPS1991-05//data/cry-00-02_data_000003.h5</li> <li>hito-CPS1991-05//data/cry-00-02_data_000004.h5</li> <li>hito-CPS1991-05//data/cry-00-02_master.h5</li> <li>hito-CPS1991-05//data/cry-00-03_data_000001.h5</li> <li>hito-CPS1991-05//data/cry-00-03_data_000002.h5</li> <li>hito-CPS1991-05//data/cry-00-03_data_000003.h5</li> <li>hito-CPS1991-05//data/cry-00-03_data_000004.h5</li> <li>hito-CPS1991-05//data/cry-00-03_master.h5</li> <li>hito-CPS1991-05//data/cry-00-04_data_000001.h5</li> <li>hito-CPS1991-05//data/cry-00-04_data_000002.h5</li> <li>hito-CPS1991-05//data/cry-00-04_data_000003.h5</li> <li>hito-CPS1991-05//data/cry-00-04_data_000004.h5</li> <li>hito-CPS1991-05//data/cry-00-04_master.h5</li> <li>hito-CPS1991-05//data/cry-00-05_data_000001.h5</li> <li>hito-CPS1991-05//data/cry-00-05_data_000002.h5</li> <li>hito-CPS1991-05//data/cry-00-05_data_000003.h5</li> <li>hito-CPS1991-05//data/cry-00-05_data_000004.h5</li> <li>hito-CPS1991-05//data/cry-00-05_master.h5</li> <li>hito-CPS1991-05//data/cry-00-06_data_000001.h5</li> <li>hito-CPS1991-05//data/cry-00-06_data_000002.h5</li> <li>hito-CPS1991-05//data/cry-00-06_data_000003.h5</li> <li>hito-CPS1991-05//data/cry-00-06_data_000004.h5</li> <li>hito-CPS1991-05//data/cry-00-06_master.h5</li> </ul> <p>&nbsp;</p> <ul> <li>#####################################################</li> <li># &#39;cry-01-??_master.h5&#39; are for &#39;full rotation helical&#39; datasets</li> <li>#####################################################</li> <li># 60.0 deg. / datasets</li> <li>#&nbsp; 0.1 deg. / frame</li> <li>#&nbsp; 600 frames / datasets</li> <li>#&nbsp; 100 frames in each data file</li> <li># ** The program could collect &#39;full rotation&#39; helical dataset</li> <li># but 60 deg. was set for this demonstration.</li> <li>#####################################################</li> <li>hito-CPS1991-05//data/cry-01-00_data_000001.h5</li> <li>hito-CPS1991-05//data/cry-01-00_data_000002.h5</li> <li>hito-CPS1991-05//data/cry-01-00_data_000003.h5</li> <li>hito-CPS1991-05//data/cry-01-00_data_000004.h5</li> <li>hito-CPS1991-05//data/cry-01-00_data_000005.h5</li> <li>hito-CPS1991-05//data/cry-01-00_data_000006.h5</li> <li>hito-CPS1991-05//data/cry-01-00_master.h5</li> </ul>