Thermolysin dataset collected with the helical scheme implemented in HEBI in the ZOO system

<p>Thermolysin is used as a standard crystal for evaluation of the automated data collection system ZOO developed at SPring-8 BL32XU.</p> <p>Both edges of a crystal with size of 30&times;30&times;220 &micro;m³ were automatically defined using&nbsp;2(width)&times;15(height) &micro;m² beam.&nbsp;</p> <p>After dose estimation with KUMA by using the crystal size,&nbsp;other X-ray parameters and data collection parameters, shutter-less helical&nbsp;data collection was applied&nbsp;along the 3D vector consisted by the defined both crystal edges.</p> <p>The intended absorbed dose for data collection was set to 8 MGy.</p> <p>The crystals belonged to space group P6₁22&nbsp;with unit cell parameters a=b=93.0, c=129.5, alpha,beta=90 gamma=120.</p> <p>Data was processed up to&nbsp;1.46 &Aring; resolution in the published result (Hirata et al. accepted) using KAMO.&nbsp;</p>