Optimisation of HTT-HAP40 purification using heparin affinity chromatography (2019/01/14)

<p><strong>Project</strong>&nbsp;- Huntingtin structure-function open lab notebook. &nbsp;</p> <p><strong>Experiment</strong>&nbsp;- Optimisation of HTT-HAP40 purification using heparin affinity chromatography.</p> <p><strong>Aims</strong> -&nbsp;The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin.&nbsp;To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems will be carried out using heparin and FLAG affinity chromatography.&nbsp;</p>