Quantitative data on expression of eve, gt and hb in early Drosophila embryo

<p>This dataset is&nbsp;supplementary to the following manuscript:</p> <p>Svetlana Surkova, Alena Sokolkova, Konstantin Kozlov, Sergey V. Nuzhdin and Maria Samsonova (2019). Quantitative analysis reveals genotype- and domain- specific differences between mRNA and protein expression of segmentation genes in <em>Drosophila</em>. <strong><em>Developmental Biology</em></strong>,&nbsp;&nbsp;doi: 10.1016/j.ydbio.2019.01.006.</p> <p>The archive contains&nbsp;recently obtained quantitative data on expression of <em>eve, gt</em> and <em>hb</em> in <em>Drosophila</em> early embryo at the level of mRNA and protein. It also includes integrated expression profiles of&nbsp;these genes, combined with the prior&nbsp;datasets (Surkova et al., 2008, Surkova et al., 2013).</p> <p>Embryos are classified into 8 time classes within cleavage cycle 14A. Genotype is specified as &ldquo;wt&rdquo; (wild type), &ldquo;het&rdquo; (<em>Kr+/Kr-</em> heterozygotes) and &quot;mut&quot; (<em>Kr</em> null mutants).</p> <p>Stained channels: EHG (<em>eve</em> mRNA, <em>hb</em> mRNA, <em>gt</em> mRNA); GGE (<em>gt</em> mRNA, Gt protein, <em>eve</em> mRNA); GE (<em>gt </em>mRNA, <em>eve</em> mRNA); ghe (<em>gt</em> mRNA, Hb protein, <em>eve</em> mRNA); HHE (Hb protein, <em>hb</em> mRNA, <em>eve</em> mRNA); GHH (<em>gt</em> mRNA, Hb protein, <em>hb</em> mRNA).</p> <p><strong>The data are&nbsp;arranged in&nbsp;three folders:</strong></p> <p><strong>1. QData_norm</strong></p> <p>Includes coordinates and fluorescence intensities of segmented nuclei for each individual embryo after subtraction of non-specific background signal.</p> <p><em>Files are arranged according to time class (T1-T8) and named according to the following scheme:</em></p> <p>&lt;stained channel&gt;&lt;number&gt;_norm.txt</p> <p><em>Structure of data file:</em></p> <p><strong>A-P-coord | D-V-coord | channel 1&nbsp;| channel 2&nbsp;| channel 3&nbsp;</strong></p> <p><strong>2. QData_registered</strong></p> <p>Registered one-dimensional gene expression profiles.</p> <p><em>Files are arranged according to time class (T1-T8) and named according to the following scheme:</em></p> <p>&lt;stained channel&gt;&lt;number&gt;_reg.txt</p> <p><em>Structure of data file:</em></p> <p><strong>Number | A-P-coord | channel 3&nbsp;| channel 2&nbsp;| channel 1</strong></p> <p><strong>3. Integrated_data</strong></p> <p>Integrated one-dimensional profiles of <em>eve, hb</em> and <em>gt</em> expression at mRNA and protein levels for wild type embryos, <em>Kr</em> mutants and <em>Kr+/Kr</em>- heterozygotes.</p> <p><em>eve </em>and <em>gt</em> profiles&nbsp;were&nbsp;normalized on stripe 1 and second anterior domain respectively (see &quot;Materials and Methods)&quot;.</p> <p><em>Files are named according to the following scheme:</em></p> <p><strong>&lt;gene&gt;&lt;genotype&gt;&lt;gene product&gt;&lt;time class&gt;_100</strong></p> <p><em>Structure of data file:</em></p> <p><strong>Number |&nbsp;A-P<em>&nbsp;</em>coord&nbsp;of an averaged nucleus | averaged intensity</strong></p> <p><em>hb profiles&nbsp;are named according to the following scheme:</em></p> <p><strong>&lt;gene&gt;&lt;genotype&gt;&lt;gene product&gt;&lt;time class&gt;</strong></p> <p><em>Structure of data file:</em></p> <p><strong>Number | A-P<em>&nbsp;</em>coord&nbsp;of an averaged nucleus | averaged intensity | standard deviation</strong></p> <p><strong>References</strong></p> <ol> <li>Surkova S., Kosman D., Kozlov K., Myasnikova E., Samsonova A.A., Spirov A., Vanario-Alonso C.E., Samsonova M., Reinitz J. (2008). Characterization of the <em>Drosophila</em> segment determination morphome. Developmental biology 313(2):844-862.</li> <li>Surkova S., Golubkova E., Manu, Panok L., Mamon L., Reinitz J., Samsonova M. (2013) Quantitative dynamics and increased variability of segmentation gene expression in the <em>Drosophila Kruppel</em> and <em>knirps</em> mutants (2013). Dev Biol. 376: 99-112. doi: 10.1016/j.ydbio.2013.01.008.</li> </ol> <p>&nbsp;</p>